BCCM/GeneCorner DNA library details

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Aspergillus niger library (LMBP LIB8)

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Library name: Aspergillus niger library
Library type: cDNA
Library accession number: LMBP LIB8
Organism: Aspergillus niger
Priming method: NotI-(dT)15 (unidirectional)
Vector name: pSCGAL10-SN
Vector sequence file: p2471.txt
Vector circular map: p2471.pdf
Vector accession number: LMBP 2471
Vector type: yeast expression vector
Cloning sites: SfiI-NotI
Library coverage: 4,0 x 10E5
Average insert size: 1,25 kb
Distributed as: pooled library DNA
DNA concentration: 200 ng/µl
Volume: 5 µl
Further information: Aspergillus niger N402 was grown in rich YPD medium, consisting of 1% bacto-yeast extract, 2% bacto-peptone and 2% dextrose.
The host strain Escherichia coli K12 MC1061 or DH5α can be used for electroporation to amplify the library. The transformation mixture can then be streaked on selective solid medium (LB-Lennox + 100 µg/ml ampicillin) and cultivated at 37°C to obtain the different library clones.
History of deposit: The cDNA library was deposited by Prof. R. Contreras1.
1 Department of Molecular Biology, Ghent University, Belgium
Library reference: Veldhuisen et al., Mol. Gen. Genet. 256 (1997), 446-455 [PMID: 9393442]
Related reference: -
Restricted distribution: - BCCM MTA

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