BCCM/GeneCorner DNA library details

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Pichia pastoris library (LMBP LIB16)

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Library name: Pichia pastoris library
Library type: cDNA
Library accession number: LMBP LIB16
Organism: Pichia pastoris
Priming method: NotI-(dT)15 (unidirectional)
Vector name: pSCGAL10-SN
Vector sequence file: p2471.txt
Vector circular map: p2471.pdf
Vector accession number: LMBP 2471
Vector type: yeast expression vector
Cloning sites: SfiI-NotI
Library coverage: 3,0 x 10E5
Average insert size: 1,19 kb
Distributed as: pooled library DNA
DNA concentration: 1 µg/µl
Volume: 5 µl
Further information: Pichia pastoris GTS115 was grown in minimal SDC medium, consisting of 0.67% bacto-yeast nitrogen base without amino acids, 2% dextrose and 0.2% cas amino acids.
The host strain Escherichia coli K12 MC1061 or DH5α can be used for electroporation to amplify the library. The transformation mixture can then be streaked on selective solid medium (LB-Lennox + 100 µg/ml ampicillin) and cultivated at 37°C to obtain the different library clones.
History of deposit: The cDNA library was deposited by Prof. R. Contreras1.
1 Department of Molecular Biology, Ghent University, Belgium
Library reference: - [PMID: -]
Related reference: -
Restricted distribution: - BCCM MTA

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