BCCM/GeneCorner DNA library details

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Mouse thymoma EL4/13.18 library (LMBP LIB15)

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Library name: Mouse thymoma EL4/13.18 library
Library type: cDNA
Library accession number: LMBP LIB15
Organism: Mus musculus
Priming method: Oligo(dT) + Random (bidirectional)
Vector name: pCAGGS
Vector sequence file: p2453.txt
Vector circular map: p2453.pdf
Vector accession number: LMBP 2453
Vector type: eukaryotic expression vector
Cloning sites: EcoRI
Library coverage: 1,3 x 10E6
Average insert size: 1,55 kb
Distributed as: pooled library DNA
DNA concentration: 150 ng/µl
Volume: 5 µl
Further information: The host strain Escherichia coli K12 MC1061 or DH5α can be used for electroporation to amplify the library. The transformation mixture can then be streaked on selective solid medium (LB-Lennox + 100 µg/ml ampicillin) and cultivated at 37°C to obtain the different library clones.
History of deposit: The cDNA library was deposited by Prof. R. Contreras1.
1 Department of Molecular Biology, Ghent University, Belgium
Library reference: - [PMID: -]
Related reference: -
Restricted distribution: - BCCM MTA

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