BCCM/GeneCorner DNA library details

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Mouse fibrosarcoma L929 R2 (TNF + cycloheximide induced) library (LMBP LIB12)

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Library name: Mouse fibrosarcoma L929 R2 (TNF + cycloheximide induced) library
Library type: cDNA
Library accession number: LMBP LIB12
Organism: Mus musculus
Priming method: Random (bidirectional)
Vector name: pGAD424
Vector sequence file: p3106.txt
Vector circular map: p3106.pdf
Vector accession number: Clontech
Vector type: yeast two hybrid vector
Cloning sites: EcoRI
Library coverage: 2,5 x 10E6
Average insert size: 1,00 kb
Distributed as: pooled library DNA
DNA concentration: 150 ng/µl
Volume: 5 µl
Further information: The host strain Escherichia coli K12 MC1061 or DH5α can be used for electroporation to amplify the library. The transformation mixture can then be streaked on selective solid medium (LB-Lennox + 100 µg/ml ampicillin) and cultivated at 37°C to obtain the different library clones.
History of deposit: The cDNA library was deposited by Prof. R. Contreras1.
1 Department of Molecular Biology, Ghent University, Belgium
Library reference: De Valck et al., FEBS Lett. 384 (1996), 61-64 [PMID: 8797804]
Related reference: Vanhaesebroeck et al., Cancer Res. 51 (1991), 2469-2477 [PMID: 1849793]
Van de Craen et al., FEBS Lett. 403 (1997), 61-69 [PMID: 9038361]
Heyninck et al., J. Cell Biol. 145 (1999), 1471-1482 [PMID: 10385526]
Restricted distribution: - BCCM MTA

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