BCCM/GeneCorner DNA library details

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Mouse thymoma EL4/13 library (LMBP LIB10)

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Library name: Mouse thymoma EL4/13 library
Library type: cDNA
Library accession number: LMBP LIB10
Organism: Mus musculus
Priming method: NotI-(dT)15 (unidirectional)
Vector name: pCAGGS-SN
Vector accession number: /
Vector type: eukaryotic expression vector
Cloning sites: SfiI-NotI
Library coverage: 3,0 x 10E5
Average insert size: 1,43 kb
Distributed as: pooled library DNA
DNA concentration: 1 µg/µl
Volume: 5 µl
Further information: The host strain Escherichia coli K12 MC1061 or DH5α can be used for electroporation to amplify the library. The transformation mixture can then be streaked on selective solid medium (LB-Lennox + 100 µg/ml ampicillin) and cultivated at 37°C to obtain the different library clones.
History of deposit: The cDNA library was deposited by Prof. R. Contreras1.
1 Department of Molecular Biology, Ghent University, Belgium
Library reference: - [PMID: -]
Related reference: -
Restricted distribution: - BCCM MTA

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